Fait partie de [OMB45]

2003 - 172 p.
p. 79-83

Occurrence of different Plum Pox Virus strains in several stone fruit species in Austria

Laimer M., Mendonça D., Arthofer W., Hanzer V., Myrta A., Boscia D.

A survey was carried out in Austria to type PPV isolates in stone fruit orchards and hedges along their borders. A total of 181 isolates were typed by serological and molecular (IC-RT-PCR and PCR/RFLP in the CP and the Nib-genes) techniques. Selected isolates (54) were tested by DASI-ELISA using the following monoclonal antibodies (MAbs): 5B (universal), 4DG5 (PPV-D specific), AL (PPV-M specific), AC (PPV-C specific) and EA24 (PPV-El Amar specific). The vast majority of Austrian PPV isolates (159) belong to D strain and only a few isolates (16) could be assigned for sure to PPV-M. For 6 isolates, the serological and molecular data for strain identification were not in agreement, which could be explained by sequencing analyses.

Mots-clés    

AUTRICHE, FRUITS A NOYAU, PCR, POTYVIRUS SHARKA DU PRUNIER, TEST ELISA, VIRUS DES VEGETAUX

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Laimer M., Mendonça D., Arthofer W., Hanzer V., Myrta A., Boscia D. Occurrence of different Plum Pox Virus strains in several stone fruit species in Austria. In : Myrta A. (ed.), Di Terlizzi B. (ed.), Savino V. (ed.). Virus and virus-like diseases of stone fruits, with particular reference to the Mediterranean region. Bari : CIHEAM, 2003. p. 79-83. (Options Méditerranéennes : Série B. Etudes et Recherches; n. 45). http://om.ciheam.org/om/pdf/b45/03001775.pdf