Fait partie de [OMB45]

2003 - 172 p.

Characterization of Hungaria Plum Pox Virus isolates

Kolber M., Németh M., Krizbai L., Ember I.

Characterization of 97 Hungarian PPV isolates was carried out by ELISA and PCR. PCR detected 59 PPV-M isolates, 25 PPV-D isolates and 15 D+M mixed infections. The serological analysis indicated 72 isolates belonging to PPV-M and 25 to PPV-D serotype. There was a reasonably good correlation between the results of ELISA and PCR. However, detection of PPV-D in mixed infections by PCR indicated the greater sensitivity of this method compared to ELISA. Contradictory results of serological and molecular characterization were obtained only in a single case.

Mots-clés    

FRUITS A NOYAU, HONGRIE, PCR, POTYVIRUS SHARKA DU PRUNIER, TEST ELISA, VIRUS DES VEGETAUX

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Kolber M., Németh M., Krizbai L., Ember I. Characterization of Hungaria Plum Pox Virus isolates. In : Myrta A. (ed.), Di Terlizzi B. (ed.), Savino V. (ed.). Virus and virus-like diseases of stone fruits, with particular reference to the Mediterranean region. Bari : CIHEAM, 2003. p. 73-78. (Options Méditerranéennes : Série B. Etudes et Recherches; n. 45). http://om.ciheam.org/om/pdf/b45/03001774.pdf