Plum and peach samples were collected from different regions in Yugoslavia and strain identification was done by using: (i) PCR-RFLP; (ii) RT PCR with strain specific primers; (iii) DASI ELISA with PPV-D and PPV-M specific Mabs; and (iv) EBIA. Results showed the existence of three groups of isolates belonging to M and D serotypes. In the first two groups, PPV-M predominated (120 of 143 isolates studied), while the remaining 23 isolates belonged to PPV-D and were scattered sporadically through the area sampled. A third group of 34 isolates that were collected in plum and apricot from central Serbia were serologically PPV-M (coat protein migration) and negative against D-specific panel of MAbs. However, all of them were found to have an Rsal restriction site on the 243 bp amplified fragment, which is typical of D isolates. Grafting experiments supported previous findings at Cacak Centre that PPV isolates from central Serbia do not infect peach.