In this study, we tested matured embryos as explants from eight Moroccan durum wheat varieties (Irden, Marzak, Kyperounda, Isly, Amria, Karim, Marouane, and Tomouh) and five induction media (M1 to M5) based on MS media (macro and oligo-elements) which differed with respect to concentrations of plant hormones (2,4-D and BA), vitamins, sucrose, maltose, L-asparagine, and solidifying agents. All tested media induced embryogenic callus for the varieties and regenerated plantlets. However, a significant effects of variety, medium and variety x medium interaction were observed for callus induction and regeneration. We used embryogenic callus derived from immature embryos for genetic transformation of HVA1 gene of barley. In this study, we identified for the first time, favorable media for induction and regeneration from mature embryo explants of Moroccan durum wheat varieties. Also, we successfully transformed durum wheat with HVA1 gene via particle bombardment. Since the transgenic plants developed in this study contained barley HVA1 gene, further analysis for tolerance to water and salt stresses in subsequent generations needs to be undertaken.