To improve the sustainability of livestock farming systems in a mountain area very close to Madrid city, Central Spain, the response of permanent pastures soil conditions to the application of biodynamic preparations, was studied after three and a half years period. Up to sixteen soil parameters (chemical, physical and biological) were measured in plots established in three commercial farms located along the altitudinal gradient present in the area. Results obtained suggest that these preparations have a positive influence on soil conditions depending on environmental conditions, being higher in more extreme environments, supporting thus, previous research done until now only on tilled soils.