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2008 - 481 p.

A transgenic approach to understanding gene expression in cereals

Jones H.D., Freeman J., Sparks C.A.

As methods for the genetic transformation and regeneration of cereals become increasingly facile and efficient, other limitations in the use of this approach become more obvious. For some transgenic applications, strong constitutive over expression is required while for others, finely targeted or inducible expression is preferable. However, there is a lack of well-defined constitutive, tissue-specific or inducible promoters for cereals. Here, we describe our work to characterize promoter sequences that drive defined patterns of expression in transgenic wheat using the GUS reporter gene.



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Jones H.D., Freeman J., Sparks C.A. A transgenic approach to understanding gene expression in cereals. In : Molina-Cano J.L. (ed.), Christou P. (ed.), Graner A. (ed.), Hammer K. (ed.), Jouve N. (ed.), Keller B. (ed.), Lasa J.M. (ed.), Powell W. (ed.), Royo C. (ed.), Shewry P. (ed.), Stanca A.M. (ed.). Cereal science and technology for feeding ten billion people: genomics era and beyond. Zaragoza : CIHEAM / IRTA, 2008. p. 427-430. (Options Méditerranéennes : Série A. Séminaires Méditerranéens; n. 81). Meeting of the Eucarpia Cereal Section, 2006/11/13-17, Lleida (Spain). http://om.ciheam.org/om/pdf/a81/00800895.pdf