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2008 - 481 p.
p. 387-389

Genetic analysis of nitrogen accumulation and protein composition in wheat kernel

Charmet G., Martre P.

The maximum rate and duration for grain dry matter (DM) and nitrogen (N) accumulation were evaluated for 194 recombinant inbred lines (RILs) from a cross between two French wheat cultivars. Grain protein composition was analysed by capillary electrophoresis (CE) and size-exclusion high performance liquid chromatography (SE-HPLC). Correlation analyses revealed that DM and N accumulation rates were closely related, and that protein composition was primarily influenced by the total quantity of N per grain. A genetic map of 254 molecular markers allowed us to identify 7 quantitative trait loci (QTL). Five QTLs were significantly associated with the kinetics of DM and N accumulation, and two of them also influenced protein composition. Two QTLs affected only the protein composition. We also used the SiriusQuality1 wheat simulation model to analyze genotype × environment interactions for grain protein composition. QTLs for the genotypic key parameters of total grain nitrogen partitioning were detected.

Mots-clés    

AMANDE, AZOTE, BLE, CARTE GENETIQUE, GENETIQUE QUANTITATIVE, MARQUEUR GENETIQUE, PROTEINE DE CEREALE, TECHNIQUE ANALYTIQUE

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Charmet G., Martre P. Genetic analysis of nitrogen accumulation and protein composition in wheat kernel. In : Molina-Cano J.L. (ed.), Christou P. (ed.), Graner A. (ed.), Hammer K. (ed.), Jouve N. (ed.), Keller B. (ed.), Lasa J.M. (ed.), Powell W. (ed.), Royo C. (ed.), Shewry P. (ed.), Stanca A.M. (ed.). Cereal science and technology for feeding ten billion people: genomics era and beyond. Zaragoza : CIHEAM / IRTA, 2008. p. 387-389. (Options Méditerranéennes : Série A. Séminaires Méditerranéens; n. 81). Meeting of the Eucarpia Cereal Section, 2006/11/13-17, Lleida (Spain). http://om.ciheam.org/om/pdf/a81/00800886.pdf