Scald, caused by the fungus Rhynchosporium secalis, is a major disease of barley (Hordeum vulgare L.). Several resistance genes and QTLs have been described, but little is known about defence mechanisms and genes involved. By combining QTL analysis with the identification and mapping of candidate genes, the project aims at the development of molecular markers for scald resistance. Overall, two QTL on chromosome 2H and 7H were detected explaining together more than 80% of the phenotypic variance. Identification of candidate genes was carried out by global expression profiling using the Affymetrix GeneChip(r) Barley Genome Array in addition to qRT-PCR analysis. Later was performed to validate newly identified candidate genes as well as potential candidates from literature. For example, differential expression of barley PR5 genes was studied and the involvement of some TLP5-isoforms was proven. However, TLP5-genes did not map in detected QTL regions. Therefore, candidate gene analysis is still ongoing.