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2008 - 481 p.

Barley Bmy1 gene intron III inter- and intra-haplotype variability

Sjakste T.G., Zhuk A.F.

The highly polymorphic barley endosperm specific b-amylase gene (Bmy1) provides an excellent model for comparative genomic analysis of the allelic variants and evaluation of the mechanism of the gene structural rearrangements. Simple alignment of the available sequences of four allelic variants of the structural gene, suggests huge number of polymorphic loci in coding and noncoding regions of the gene. Some of them could influence enzymatic properties or/and could be used for more precise variety discrimination. Interesting data were accumulated on the intron III polymorphisms. We described several linkage blocks on three polymorphic events (1+6 bp indel, MS size and C698.T variation) that are transmitted through the generations of different independent pedigrees. Our analysis suggested the existence of certain associations between MS locus variability and other Bmy1 polymorphisms. Therefore in our following work (Sjakste and Zhuk, 2006) we tried to obtain more general view on Bmy1 intron III polymorphisms in different barley varieties and to develop approaches for more precise haplotype classification and characterization of inter- and intra-haplotype variability. Here we would like to present short up to date review of our data.



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Sjakste T.G., Zhuk A.F. Barley Bmy1 gene intron III inter- and intra-haplotype variability. In : Molina-Cano J.L. (ed.), Christou P. (ed.), Graner A. (ed.), Hammer K. (ed.), Jouve N. (ed.), Keller B. (ed.), Lasa J.M. (ed.), Powell W. (ed.), Royo C. (ed.), Shewry P. (ed.), Stanca A.M. (ed.). Cereal science and technology for feeding ten billion people: genomics era and beyond. Zaragoza : CIHEAM / IRTA, 2008. p. 49-52. (Options Méditerranéennes : Série A. Séminaires Méditerranéens; n. 81). Meeting of the Eucarpia Cereal Section, 2006/11/13-17, Lleida (Spain). http://om.ciheam.org/om/pdf/a81/00800801.pdf