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Genetic variability analysis of a collection of old Portuguese bread wheat using ISSRs
Carvalho A., Lima-Brito J., Maçãs B., Guedes-Pinto H.
Forty-eight bread wheat cultivars of an old Portuguese collection using ISSR markers were analysed. We used 18 ISSR primers that amplified a total number of 245 ISSR loci, being 233 of them polymorphic. Considering the overall analysis, the percentage of ISSR polymorphism was lower, 40.6 percent, since different primers amplified ISSR fragments with the same molecular weight in all the cultivars. After clustering analysis, we verified that most cultivars belonging to the same botanical variety were clustered in the same main group. However, an intra-variety ISSR polymorphism was also observed.
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BLE TENDRE, MARQUEUR GENETIQUE, TRITICUM AESTIVUM, VARIATION GENETIQUECiter cet article
Carvalho A., Lima-Brito J., Maçãs B., Guedes-Pinto H. Genetic variability analysis of a collection of old Portuguese bread wheat using ISSRs. In : Molina-Cano J.L. (ed.), Christou P. (ed.), Graner A. (ed.), Hammer K. (ed.), Jouve N. (ed.), Keller B. (ed.), Lasa J.M. (ed.), Powell W. (ed.), Royo C. (ed.), Shewry P. (ed.), Stanca A.M. (ed.). Cereal science and technology for feeding ten billion people: genomics era and beyond. Zaragoza : CIHEAM / IRTA, 2008. p. 35-38. (Options Méditerranéennes : Série A. Séminaires Méditerranéens; n. 81). Meeting of the Eucarpia Cereal Section, 2006/11/13-17, Lleida (Spain). http://om.ciheam.org/om/pdf/a81/00800798.pdf