Spring durum wheat cultivars were evaluated under conditions of Prague locality for two years (1997 and 1998) and compared with check bread wheat cultivars Sandra and Munk. Grain quality parameters were evaluated in addition to characters like phenological characteristics, response to diseases, spike characters, yield components and yield estimation.Semiautomatic Kjeldahl method (Kjeltec Auto 1030 Analyzer) was used for crude protein evaluation and Glutomatic 2200 system for wet gluten content and gluten index (GI). In crude protein and wet gluten contents, the durum wheat accessions exceeded bread wheat cultivars. The highest quality parameters (17,0 per cent of crude protein and 41.3 per cent of wet gluten content) were recorded for Bejah cultivar. All quality characteristics were strongly influenced by different conditions of year.