Our study focused on reverse genetics for the characterization of genes identified as likely to increase tolerance of wheat to salinity. Salt tolerance comes from genes that limit the rate of salt uptake by the plant from the soil and the transport of salt throughout the plant, adjust the ionic and osmotic balance of cells in roots and shoots, and regulate leaf development and the onset of senescence. Salt-tolerant candidate genes families in wheat and other cereals such as HKT, NHX, SOS, and HAK were identified and downloaded from the NCBI and wheat genomics of abiotic stress (WGAS) databases. Comparative studies and analyses, such as search for domains, multiple sequence alignments and phylogenetic tree constructions as well as primers design were carried out using bioinformatics tools. Sixty candidate genes for salt-tolerance were identified. Several protein domains have been characterized: (i) the protein family of HKT genes contains a TrKH (cation transport protein) domain, (ii) the protein family of NHX genes contains a Na+/H+ exchanger domain, (iii) a Ktrans (K+ potassium transporter) domain for the protein family of HAK genes, (iv) a Na+/H+ exchanger domain and a cNMP (cyclic nucleotide binding domain) for SOS1 genes, (v) a serine/threonine protein kinase domain for SOS2 genes, and (vi) an EF-hand (calcium binding protein) domain for SOS3 genes. Multiple sequence alignments of HKT genes in wheat revealed a high frequency of glycine and serine amino acids conserved in the consensus sequence. According to the phylogenetic tree analysis, HKT genes were grouped into two subfamilies, and this division is associated with a substitution of a glycine/serine residue intended to be in first loop pores of the protein. All members of the subfamily 1 have a serine at this position, whereas members of subfamily 2 (except OsHKT1) have a glycine. The RT-PCR primers associated with the candidate genes for the studied trait were designed as markers for selection to assist the cereal breeding program.