TY - CHAP AU - Miroshnichenko D. AU - Filippov M. AU - Dolgov S. PY - 2008/// TI - Genetic engineering of russian wheat genotypes for abiotic stress resistance T2 - Cereal science and technology for feeding ten billion people: genomics era and beyond PB - Zaragoza : CIHEAM / IRTA VL - 81 T3 - Options Méditerranéennes : Série A. Séminaires Méditerranéens SP - 205-207 A2 - Christou P. A2 - Graner A. A2 - Jouve N. A2 - Keller B. A2 - Lasa J.M. A2 - Molina-Cano J.L. A2 - Royo C. A2 - Shewry P. A2 - Stanca A.M. A2 - Hammer K. A2 - Powell W. AB - The biolistic transformation protocol allowing generating a number of independent transgenic wheat plants has been worked out. A complete process from the introduction of foreign DNA into wheat cells to the field trials have been developed for several Russian wheat cultivars. Homozygous T2-T4 transgenic wheat progeny constitutively expressing herbicide resistance gene (bar) and soil salinity resistance gene (hvnhx2) were generated. Field tests of T3/T4 homozygous offspring demonstrated that agronomic traits and yields of transgenic lines treated with herbicide (1.0 percent Basta) and non-treated transgenic/non-transgenic plants are comparable or higher for transgenic lines. Transgenic wheat plants expressing gene of barley Na+/H+ vacuolar antiporter were able to grow, flower, and produce seeds in the presence of 200 mM sodium chloride. In the greenhouse the growth of the T1 transgenic wheat plants in saline conditions was about 80 percent of the same in non-saline conditions, while the non-transgenic progeny showed 50 percent delay in the growth. N1 - Meeting of the Eucarpia Cereal Section, 2006/11/13-17, Lleida (Spain) UR - http://om.ciheam.org/om/pdf/a81/00800841.pdf ER -