Calli formation and plant regeneration from dicotyledonous species were successfully and widely reported. However, for monocotyledonous species (cereal), this success did not have the same level. This study highlights a rapid technique of wheat plant regeneration based on in vitro culture of young spike explants. This technique could be used to exert selection pressure to abiotic stress or to transfer genes of agronomic interest and produce transgenic plants. It shows that callus induction and development is under genotype and medium control. This technique is faster and less exposed to contamination than immature embryo methods.