Plum pox potyvirus EL Amar strain was detected in naturally infected apricot, peach and plum trees showing chlorotic rings, pale green lines and spots on the leaves, pale yellow rings and deformation on the fruits and typical spots on the stones collected from different locations of Egypt. Symptom observations followed by DAS-ELISA were used to identify the presence of PPV. Strain identification was done by RT-PCR, IC-RT-PCR, RFLP and sequence analysis using primers specific for PPV-C (cherry), PPV-D (Dideron), PPV-M (Marcus) and El-Amar strains. Results indicated that PPV-EA (El-Amar) is the only strain of the virus found in Egypt.