Leaf analysis is a suitable technique to evaluate the nutritional status of forest stands since it reflects both the availability of nutrients in the soil and the ability of the trees to absorb them. This study aims to assess the effect of some nutrients on the needle mineral composition of Pinus pinea L. one year after their application to the soil. The experiment was established on a stone pine planted in 2009, located in the Portuguese Region of Ribatejo on a soil with acid reaction, medium levels of organic matter and extractable potassium, and low levels of extractable phosphorus, manganese, zinc, copper and boron. Eight experimental treatments consisting of different combinations of nitrogen, phosphorus, potassium, micronutrients, and limestone were considered. The needles were collected in March from the middle part of the growths of the former year. Fertilization induced significant differences (p ≤ 0.05) on the needle levels of nitrogen, phosphorus, sulphur, manganese, zinc and boron. The application of phosphorus and boron increased the foliar levels of these nutrients, but liming induced lower levels of manganese and zinc. Concerning nitrogen, the levels of soil organic matter may be responsible for the appropriate nitrogen levels in the needles. The study will continue for further years in order to confirm the obtained results.