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2008 - 481 p.

Genetic diversity of Fusarium head blight QTLs among Western European wheat

Zwart R.S., Muylle H., van Bockstaele E., van Huylenbroeck J., Roldán-Ruiz I.

To aid the challenge of breeding FHB resistant wheat we are investigating the genetic diversity of resistance genes controlling FHB in European wheat germplasm. Field trials were conducted to measure two types of resistance: to initial infection and to spread. Overall (combined type I and type II) resistance and type I resistance were assessed in artificial spray inoculation trials conducted over three consecutive years. All plots were inoculated with a mixed conidial suspension of F. graminearum and F. culmorum, three times at three day intervals commencing at the date of ear emergence for over 50 percent of the plots. Plots were rated 22 days from the date of ear emergence for percent FHB severity and incidence. Type II resistance of the wheat lines were assessed in replicated point-inoculation trials. Field trials were conducted in 2006 at two locations with two replications at each location. Ten heads per plot were inoculated at anthesis by injecting 10ul of a 100,000 conidia/ml suspension into the fifth spikelet from the tip of the head. A split-plot design was used with main plots (wheat genotypes) consisting of two sub-plots (Fusarium isolate). Two highly aggressive Fusarium isolates were used: F. graminearum IFA 65 and F. culmorum IFA 104. Disease symptoms were evaluated on days 10, 14, 18, 22, and 26 after inoculation and an area under the disease progress curve (AUDPC) was calculated.

Mots-clés    

BLE, EUROPE OCCIDENTALE, FUSARIUM, RESISTANCE AUX MALADIES, RESISTANCE GENETIQUE, TRITICUM, VARIATION GENETIQUE

Citer cet article    

Zwart R.S., Muylle H., van Bockstaele E., van Huylenbroeck J., Roldán-Ruiz I. Genetic diversity of Fusarium head blight QTLs among Western European wheat. In : Molina-Cano J.L. (ed.), Christou P. (ed.), Graner A. (ed.), Hammer K. (ed.), Jouve N. (ed.), Keller B. (ed.), Lasa J.M. (ed.), Powell W. (ed.), Royo C. (ed.), Shewry P. (ed.), Stanca A.M. (ed.). Cereal science and technology for feeding ten billion people: genomics era and beyond. Zaragoza : CIHEAM / IRTA, 2008. p. 87-89. (Options Méditerranéennes : Série A. Séminaires Méditerranéens; n. 81). Meeting of the Eucarpia Cereal Section, 2006/11/13-17, Lleida (Spain). http://om.ciheam.org/om/pdf/a81/00800811.pdf