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2008 - 481 p.

Survey of molecular diversity at nud locus in barley

Barabaschi D., Laidò G., Francia E., Tondelli A., Gianinetti A., Stanca A.M., Pecchioni N.

Naked caryopsis trait in barley is controlled by a single recessive gene, nud, located on chromosome 1 (7H); it prevents adhesion of the lemma and palea to the caryopsis (hulless barley). To identify new markers closely linked to nud, to facilitate marker-assisted selection of the naked trait, we applied the RAPD protocol coupled with the Bulked Segregant Analysis strategy on the barley Proctor (hulled) x Nudinka (naked) mapping population. We identified two RAPD markers and only the most tightly linked, was successfully converted into a more specific SCAR marker. This together with other 6 markers were mapped onto a reference map. A naked/hulled barley germplasm was collected to individuate the haplotype structure surrounding the nud locus, employing most tightly linked markers. The response to leaf stripe disease was evaluated among a subset of the same germplasm, in order to investigate any possible associations between disease incidence and other recorded traits.

Mots-clés    

GENE RECESSIF, HORDEUM, MARQUEUR GENETIQUE, ORGE, PHENOTYPE, RAPD, RESISTANCE AUX MALADIES

Citer cet article    

Barabaschi D., Laidò G., Francia E., Tondelli A., Gianinetti A., Stanca A.M., Pecchioni N. Survey of molecular diversity at nud locus in barley. In : Molina-Cano J.L. (ed.), Christou P. (ed.), Graner A. (ed.), Hammer K. (ed.), Jouve N. (ed.), Keller B. (ed.), Lasa J.M. (ed.), Powell W. (ed.), Royo C. (ed.), Shewry P. (ed.), Stanca A.M. (ed.). Cereal science and technology for feeding ten billion people: genomics era and beyond. Zaragoza : CIHEAM / IRTA, 2008. p. 31-33. (Options Méditerranéennes : Série A. Séminaires Méditerranéens; n. 81). Meeting of the Eucarpia Cereal Section, 2006/11/13-17, Lleida (Spain). http://om.ciheam.org/om/pdf/a81/00800797.pdf