Stomata are found on most aerial surfaces of plants, including the epidermis, stems and flowers. Stomata control the loss of water and the exchange of gas and thus control water relations and metabolism. Differences in leaf features and stomata distribution among the species and cultivars growing in the same environment are important aspects of their environmental physiology. For this reason, stomata number was determined on the leaf epidermis. This study has been designed to obtain knowledge on stomatal size and distribution in Pistacia species and cultivars. Leaf samples were collected from different species and cultivars growing in Manisa-Yunt Mountain. There were some variations among the species and cultivars.