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Conservation of almond germplasm by cryopreservation
Shoot tips of two almond scion cultivars, 'Ne Plus Ultra' and 'Nonpareil 15-1', and an almond-peach hybrid rootstock were successfully cryopreserved using a one-step vitrification technique. Shoot tips, 2-2.5 mm long, were excised, cold-hardened, precultured, and dehydrated in a vitrification solution adopted from Sakai et al. (1990). They were then stored under liquid nitrogen (LN) for up to 6 months. After thawing and culturing the shoot tips, shoot regeneration was usually observed within 2-3 weeks. Shoot survival was 77, 52, and 78 per cent for 'Ne Plus Ultra', 'Nonpareil 15-1' and the hybrid rootstock respectively. All shoots had the same morphological appearance as the initial in vitro cultures. However, the possibility of genetic change during long-term preservation is being investigated.
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AMANDE, CONGELATION, CONSERVATION BIOLOGIQUE, VITRIFICATIONCiter cet article
Channuntapipat C., Collins G., Sedgley M. Conservation of almond germplasm by cryopreservation. In : Ak B.E. (ed.). XI GREMPA Seminar on Pistachios and Almonds. Zaragoza : CIHEAM, 2001. p. 101-106. (Cahiers Options Méditerranéennes; n. 56). 11. GREMPA Seminar on Pistachios and Almonds, 1999/09/01-04, Sanliurfa (Turkey). http://om.ciheam.org/om/pdf/c56/01600160.pdf