To succeed in the production of doubled-haploid plants of durum wheat (Triticum durum. L), gynogenesis was studied in six durum wheat varieties Anwar (AN), Jawhar (JW), Yasmine (YS), Belbachir (BB), Sebou (SB) and Kyperonda (KP). The MS medium (Murashige and Skoog 1966) supplemented with 9% sucrose and 0.8% agar was used as basal medium. 2mg /l of three auxins [2,4-D (2,4-dichloro- phenoxy acetic acid), NAA (naphthalene acetic acid), and 2.4.5-T (trichlorophenoxy acetic acid)] were added to the induction medium. The embryogenic calluses were transferred onto regeneration medium R9 without growth regulators for two weeks then the same medium supplemented with 2 mg /l BAP (benzyl adenine purine) and 0.1mg /l NAA. The results showed that 2,4-D was the most reactive auxin for all varieties studied. The effect of genotype was significantly very marked in the presence of the three auxins tested. The best rate of embryogenic callus induction was 63% obtained from the Anwar variety. The presence of light (16h per day) favorably affected the kinetics of appearance of gynogenetic embryogenic callus. The first results appeared in the second week of culture whereas, in darkness, this period lasted up to nine weeks. The study also showed a genotypic effect on the regeneration phase with the best rate (27%) obtained with the variety Anwar.